Introduction
The collection of trillions of microbes which include bacteria, fungus, viruses, archaea and eukaryotes are called human microbiota. These tiny organisms maintain a symbiotic relationship with the human body. The gut harbors the largest collection of microbes in the body that are collectively called as the gut microbiota. These microbes continuously evolve throughout the life span of an individual adapting themselves to the external and internal changes. A number of factors like the mode of birth, type of feeding, genetics, lifestyle, race, health status, exposure to medicines, environmental factors such as pollutants, noise, weather conditions, hygiene and aging influence the microbial framework in an individual.
The gut microbial ecosystem is dominated by bacteria and it is estimated that about 500-1000 species harbor the gut. A healthy gut is colonized by beneficial microbes which includes the bacteria that come under the phylum Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Fusobacteria and Verrucomicrobia. Some of the dominant members of the Firmicutes phylum include Lactobacillus, Enterococcus, Clostridium, Veillonella, Roseburia, Faecalibacterium and Ruminococcus. The predominant members of the Bacteroidetes phylum include Bacteroides and Prevotella. The phylum Actinobacteria is dominated by Bifidobacterium whereas Akkermansia muciniphila is the main bacteria of the phylum Verrucomicrobia.
These numerous microbes that are part of the gut microbial ecosystem play a vital role in maintaining the overall health and well being of an individual. Hence the gut is also regarded as a metabolic organ and the second brain. The bi-directional communication which the gut bacteria have established with all the organ and organ systems greatly influences their normal functioning. The production of metabolites, nutrients, chemical messengers as well as strengthening the gut and the overall immunity carried out by the gut microbes is crucial in maintaining the homeostasis i.e., body’s internal balance.
Any imbalance in the gut microbial ecosystem causes disturbed homeostasis resulting in disease process. Hence promoting a healthy and diversified gut bacteria is critical in terms of the health status of an individual. One of the dietary strategies to encourage the growth and development of the healthy gut bacteria is to increase the consumption of a specific nutrient called prebiotics.
Prebiotics concept
The habit of consumption of the fermentable dietary fibers has been known since thousands of years. There have been reports about the excessive consumption of lactose to enrich the intestinal bacteria. The notion that specific types of nutrients like carbohydrates can improve the gut bacteria has been known for a long time, well before the definitions of these nutrients were put forward. The scientific research conducted in the 1950s bought into light a type of factor called ‘bifidus factor’ present in human milk. This compound promoted the growth of Bifidobacterium in infants. Further scientific studies on this compound revealed the presence of oligosaccharides and glycans (also called polysaccharides) which are different types of carbohydrates. The oligosaccharides were also detected in cow’s milk. But there was no clarity regarding their physiological function.
In the later years, compounds such as inulin and other oligosaccharides produced from sucrose, galactose and xylose were identified as having bifidogenic properties i.e., those having the ability to enhance the growth of Bifidobacterium. But it was only in the year 1995 the concept of prebiotics came into existence which was put forth by Glenn Gibson and Marcel Roberfroid in their publication named “Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics “. According to them ‘prebiotics are a specific type of food ingredient that are non-digestible, but are capable of encouraging the growth and colonization of a particular or a certain number of colonic bacteria that are beneficial for the health’. Though this concept is scientifically acknowledged, some confusions on this term still exist. However, the evolution of the prebiotics concept has greatly influenced the scientific knowledge about the gut health.
With the growing list of compounds being recognized as having prebiotic potential, the research continued into identifying the specific microbes in the gut whose growth was enhanced by the use of prebiotics. It was consistently observed that prebiotics selectively increased the beneficial bacteria like Lactobacillus and Bifidobacterium. In the later studies that followed, it was interesting to note that the beneficial effects of prebiotics extended to increasing the numbers of Faecalibacterium and Akkermansia muciniplila. While it is presumed that more beneficial bacteria may also be enhanced by prebiotics, more studies with regards to the bacterial sequencing are warranted.
In the light of these observations, an expanded definition for prebiotics was put forward by researchers. According to this, prebiotics were regarded as ‘undigested dietary carbohydrates’ which are fermented by the gut bacteria producing short chain fatty acids. The recent definition of prebiotics was put forward in 2016 by International Scientific Association for Probiotics and Prebiotics (ISAPP) by an expert committee in the fields of microbiology, nutrition and clinical research. Their definition of prebiotics is “a substrate that is selectively utilized by the host microorganisms conferring a health benefit”. So, the overall picture of the prebiotic concept is the presence of three components i.e., the compound, its health beneficial effects and gut microbial enhancement.
With evidence supporting the presence of a direct link between the presence of a diversified gut bacteria and overall health, and with the increasing number of compounds being labelled as prebiotics, it is obvious that consuming a wide range of foods having prebiotic properties are needed to support the growth of healthy gut bacteria.
Categories explained
A compound can be considered as a prebiotic if it satisfies the following criteria
*Tolerant to the stomach acid.
*Does not undergo chemical breakdown due to the action of digestive enzymes.
*Is not absorbed during digestion.
*Undergoes fermentation by the gut microbes.
*Enhances the growth of the healthy gut bacteria.
*Has favorable effects on the overall health and well-being.
Among the several types of prebiotics that exist, most of them fall under the carbohydrate group and are called oligosaccharide carbohydrates. Initially the prebiotics included fermentable carbohydrates such as soluble fibers, fructooligosaccharides (FOS), galactooligosaccharides (GOS), human milk oligosaccharides (HMOs), inulin, galactans, fructans and lactulose. However, owing to the advancements in the concept and scientific research, other substances such as resistant starch (RS), pectin, polyphenol compounds like those present in curcumin (turmeric), quercetin (plant pigment present in apples, berries, citrus fruits, onions) and polyunsaturated fatty acids (PUFA) have been proposed to have prebiotic potential.
Carbohydrates
Oligosaccharides
Oligosaccharides and polysaccharides are a group of complex carbohydrates that have a prebiotic potential. They are made up of simple sugars that are bonded together. The simple sugar in question may be either glucose, fructose or galactose. The main difference between the two lies in the number of sugar units they are made of. While the oligosaccharides are made up of two or more sugar units, polysaccharides contain more than ten sugar units. Some of the examples of oligosaccharides include inulin type fructans, galactooligosaccharides, arabinoxylans and human milk oligosaccharides. The compounds such as beta-glucans, isometodextrin and resistant starch come under polysaccharides.
Fructans
Fructans are the general term used to describe the naturally occurring non-digestible carbohydrates present in foods that are derived from plants. They are mainly made up of fructose polymers i.e., many fructose molecules that are bonded together. However, they are different from fructose. The main difference lies in the number of fructose molecules. While the fructose can be digested and absorbed by the body, fructans on the other hand are non-digestible but are fermented by the gut microbes mainly colonic bacteria.
Fructans form an important part of everyday diet as they are contained in many plant foods. The plants that contain high amount of fructans have found many uses such as food, animal feed and folk medicines since ancient times. Apart from being a food component, fructans also exhibit antioxidant, immunomodulatory (a substance that modifies the immune system either by enhancing or suppressing it), anti-inflammatory, anticancer, antiallergic, antihyperglycemic (an agent that reduces the high blood sugar levels) and prebiotic properties. They also increase the absorption of minerals like calcium, iron and magnesium from the intestine.
Classification
There are five classes of fructans depending on the differences in the chemical structure. They are inulin-type fructans, levan-type fructans, graminans, inulin neoseries and mixed-type levans. The inulin-type fructans regarded as oligo or polysaccharides are the most extensively researched among the fructans. They include inulin, fructooligosaccharides and oligofructose. The main difference between these fructans lies in the number of the fructose molecules with inulin regarded as a longer chain molecule and the other two as short-chain molecules. In order to obtain the long chain inulin, they need to be physically separated while the other two can be either extracted from the plants or produced from glucose and sucrose by a chemical reaction called hydrolysis.
Applications
The longer chain inulin is less soluble and has a creamy texture whereas the short chain fructooligosaccharide is easily soluble having similar qualities to sugar. Due to these properties fructans have found application in pharmaceuticals and food industries like the production of baked foods, beverages, dairy products and food preservatives. They have been used as a sugar substitute in the production of low-calorie foods. They can be used as a replacement for fats in food production due to its ability to impart a creamy texture and gelling (property of a substance to become solid). Their applications have also been extended to producing aerated desserts, ice creams, spreads and sauces due to their stabilizing properties. They also exhibit humectant effects (retain moisture) and hence considered to offer protection against harmful microbes.
Sources
Inulin is present in a number of food plants and is stored in stems and underground tissues. Some of the plants that are good sources of fructans include onions, shallots, scallions (green or spring onions) barley, Brussels sprouts (baby cabbage), cabbage, broccoli, pistachio, garlic, wheat, chicory, asparagus (thin long green spear shaped vegetable), leek (long white vegetable with green leaves at the top that has the smell of an onion) Jerusalem artichoke (a crunchy root vegetable that resembles a ginger root), agave (a plant with large succulent leaves) and bananas.
Biological actions
Intestinal health
The inulin-type fructans have been regarded as functional foods due to their health benefits. A functional food means “a natural conventional food consumed everyday that is beneficial to the health in addition to their basic nutritive value, enhancing the overall health and well-being of an individual in addition to reducing the risk of disease and improving the quality of life”. They are considered as an important dietary fiber that positively influences the intestinal and overall health.
The colon or the large intestine has been increasingly identified as an organ that is crucial for maintaining the overall health and protection against diseases. The inulin-type fructans have been shown to positively influence the functioning of the colon and reduce the risk of diarrhoea, constipation, infections, inflammatory bowel diseases (IBD), irritable bowel syndrome (IBS) and cancer.
The inflammatory bowel disease (IBD) is a collective term used to describe the chronic inflammatory disorder of the gastrointestinal tract affecting both the small as well as the large intestine. IBD is classified into types namely ulcerative colitis (UC) and Crohn’s disease (CD). Though symptomatically both these conditions have common features such as abdominal pain, diarrhoea, rectal bleeding and weight loss, there are some differences between these two conditions. While ulcerative colitis predominantly affects the mucosal (inner) lining of the colon, Crohn’s disease can affect any part of the gastrointestinal tract though commonly affects the ileum, the last part of the small intestine.
In one of the studies conducted in individuals with ulcerative colitis, it was observed that the administration of inulin-type fructans brought about reductions in the colonic inflammation. This effect was due to the modifications of the gut bacteria by the inulin-type fructans wherein an increased population of butyrate producing bacteria were noted. The short chain fatty acid butyrate produced by the gut bacteria has anti-inflammatory effects on the colonic cells. On the other hand, evidence from the human studies conducted on the effects of inulin-type fructans in individuals with Crohn’s disease is not convincing though recent studies have pointed to their potential in preventing the Crohn’s disease in those at risk. However, larger studies are needed to ascertain the benefits of inulin- type fructans in individuals with UC and CD.
The above-mentioned effects of inulin-type fructans on the gut bacteria and butyrate production have also been recognized to protect against colon cancer especially sourced from chicory root and Mexican agave. However recent studies have revealed that consumption of fibers from whole grain foods was more favorable in reducing the cancer risk compared to fibers from fruits.
Gut microbial balance
It is a well-known fact that a balanced gut microbial ecosystem is crucial for maintaining the body’s internal balance. The inulin and oligofructose have been acknowledged as having pre-biotic properties in line with the numerous studies conducted. They reach the colon (large intestine) in an undigested state wherein they are fermented and exert their prebiotic effects. Reports from the studies conducted on the influence of inulin and oligofructose on the gut microbes have revealed increase in the numbers of health promoting bacteria such as Bifidobacterium and Lactobacillus. Also, increased population of butyrate producing bacteria namely Clostridium, Eubacterium and Faecalibacterium have been reported. Butyrate is a type of short chain fatty acid, a metabolite produced by the gut bacteria which is a major source of energy to the colonic cells.
Recent studies conducted on the effects of inulin type fructans in individuals with diabetic nephropathy have revealed their effectiveness in increasing the population of Akkermansia muciniplila. The increased production of short chain fatty acids especially acetate by this bacterium has been found to regulate the blood glucose levels and protect the kidneys against damage. Thus, inulin type fructans safeguard the kidneys against damage by modifying the gut microbial composition.
Bowel habit regulation
One of the basic functions of the colon is stool production and excretion. Inulin type fructans have been shown to regulate the bowel habits in addition to improving the stool mass and consistency. These effects were noted in several studies conducted on individuals with chronic constipation. This is regarded as one of the typical effects of the dietary fiber and recent studies conducted on children between the ages of 2-5 years with functional constipation (a condition commonly seen in children characterized by irregular bowel movements and hardening of stools), have acknowledged improvement in the bowel habits with the addition of inulin-type fructans.
Irritable bowel syndrome (IBS) is one of the most frequently encountered functional gastrointestinal disorder worldwide causing much distress and discomfort. Abdominal pain, bloating, altered bowel habits (constipation/diarrhoea/or both) without any obvious underlying causes are some of the features of IBS. Though the exact mechanisms behind IBS is incompletely understood, it is thought to be due to the disturbances in the gut-brain axis i.e., the reciprocal connection between the gut and the brain. In one of the recent studies that explored the effect of inulin-type fructans in individuals with IBS having constipation, it was found that there was an improvement in the overall bowel function as well as the quality of life. Despite the clearly evident benefits, more studies are needed for stronger evidence.
Strengthens gut barrier
One of the most crucial defense systems in the body is the intestinal or the gut barrier. It constitutes the largest surfaces that forms an intersection between the human body and the external environment. The gut barrier system comprises of mucus layer which is the inner slimy lining of the intestines, friendly gut microbes and a single layer of specialized epithelial cells that are tightly packed. The gut barrier selectively allows the passage of nutrients while protecting the gut from coming into contact with the harmful substances. The inulin-type fructans have been shown to strengthen the mucosal lining in addition to modifying the mucin, a special protein which is present in the mucus. This activity enhances the ability of the gut to prevent the growth of harmful microbes and the dislocation of the gut bacteria into the systemic circulation.
Balances the gut hormones
The enteroendocrine system consists of a group of specialized cells called enteroendocrine cells that are scattered throughout the gastrointestinal tract. These cells produce hormones that regulate the functioning of the gut, food intake, absorption of the nutrients, metabolism and post-prandial glucose levels. The GLP-1 mainly stimulates the production of insulin and controls the blood sugar fluctuations following a meal. The hormone ghrelin also called as the ‘hunger hormone’ regulates the appetite and food intake in addition to maintaining the blood glucose levels, protecting the heart, maintaining the muscle and bone mass.
Preliminary studies exploring the effect of inulin-type fructans on the production of hormones especially glucagon-like-peptide-1 (GLP-1) and ghrelin have revealed the effectiveness of inulin-type fructans in regulating these hormones thus balancing the appetite and blood glucose levels. In line with this observation, recent studies have put forth the potential of inulin-type fructans supplements in the management of prediabetes, type 2 diabetes, overweight and obesity. However, more studies are needed to ascertain this.
Improves metabolic health
The inulin-type fructans have been shown to bring about improvements in the blood lipid profile. Studies have demonstrated the effectiveness of inulin in bringing about reductions in the triglyceride levels. But the reports on the cholesterol levels have been inconsistent. Recent studies comparing the effects of inulin in different groups of individuals have revealed consistent reductions in the low-density lipoprotein (LDL, bad cholesterol)) levels whereas increases in the high-density lipoprotein (HDL, good cholesterol) and the total cholesterol levels were observed in individuals with prediabetes and diabetes. These studies also reported similar observations with regards to the blood glucose levels also. Though more long-term studies are needed, these findings provide insights to the potential of inulin type fructans in improving the metabolic health.
Galacto-oligosaccharides (GOS)
Image by mymedblog.org
Super